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Analysis of MCP-2 (Non-human primate) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0401-LGZ)

Introduction

Monocyte chemotactic protein 2(MCP-2; CCL8) is a protein encoded by the CCL8 gene. It is a small cytokine belonging to the CC chemokine family. MCP-2 protein is produced as a precursor containing 109 amino acids, which is cleaved to produce mature MCP-2 containing 75 amino acids. Its gene is encoded by three exons, and it chemotaxis and activates many different immune cells, including mast cells, eosinophils and basophils (involved in allergic reactions), as well as monocytes, T cells and NK cells involved in inflammatory responses.




Principle

Detection Technique: MSD Hypersensitive Multi-Factor Electrochemiluminescence
Capture Antibody: Mouse Monoclonal
Detection Antibody: Rabbit Polyclonal
Recombinant Standards: Expressed in E. coli

Applications

Cytokines & Chemokines, Immunology/Inflammation

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Serum, Plasma, Cell Culture Supernatant
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