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Analysis of MCP-4 (Non-human primate) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0405-LGZ)

Introduction

Monocyte chemotactic proteins (MCPs) are a series of cytokines with similar structure, molecular mass about 8-10kD, and chemotactic function. They all contain 4 cysteines and form two internal disulfide bonds, and belong to the CC subfamily (β subfamily) of chemokines, including MCP-1, MCP-2, MCP-3, MCP-4, MCP-5. MCPs and their receptors play important roles in immune regulation, organogenesis, modulation of hematopoiesis, and neuronal communication. MCP-4 (CCL13) is a potent eosinophil-attractant factor.




Principle

Detection Technique: MSD Hypersensitive Multi-Factor Electrochemiluminescence
Capture Antibody: Mouse Monoclonal
Detection Antibody: Mouse Monoclonal
Recombinant Standards: Expressed in E. coli

Applications

Cytokines & Chemokines, Immunology/Inflammation

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Serum, Plasma, Urine, Cell Culture Supernatant
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