Analysis of MEF2D Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0424-LGZ)

Introduction

Official Full Name: myocyte enhancer factor 2D<br />This gene is a member of the myocyte-specific enhancer factor 2 (MEF2) family of transcription factors. Members of this family are involved in the control of muscle and neuronal cell differentiation and development and are regulated by class II histone deacetylases. In acute lymphoblastic leukemia cell lines, the encoded protein was found to be fused to deleted azoosperm-associated protein 1 (DAZAP1) as a result of a translocation, suggesting a role in leukemogenesis. The encoded protein may also be involved in Parkinson's disease and myotonic dystrophy. Alternative splicing results in multiple transcript variants.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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