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Analysis of MKI67 / Ki-67 / MIB-1 by ELISA (CAT#: STEM-MB-1556-LGZ)

Introduction

KI-67, also known as Ki-67 or MKI67, is a protein encoded by the MKI67 gene in humans. Ki-67 is a 350-400 kDa nuclear protein and a member of mitosis related proteins of chromosomes. In addition, Ki-67 is associated with ribosomal RNA transcription, and its inactivation inhibits ribosomal RNA synthesis. Ki-67 interacts with 160kDa Hklp2, which promotes centrosome separation and spindle bipolarity. It also interacts directly with NIFK and binds to UBF, thus playing a role in ribosomal RNA synthesis. Ki-67 represents a highly proliferative subgroup of patients with ER-positive breast cancer, allowing them to benefit more from adjuvant chemotherapy.




Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Oncology & Cancer

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma, cell culture supernatant and other biological samples
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