Analysis of MMP23B Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1360-LGZ)

Introduction

Official Full Name: matrix metallopeptidase 23B
Also known as: MIFR; MMP22; MIFR-1; MMP23A
This gene (MMP23B) encodes a member of the matrix metalloproteinase (MMP) family, and it is part of a duplicated region of chromosome 1p36.3. Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. This gene belongs to the more telomeric copy of the duplicated region.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Rapid EGFR Mutation Analysis of Fine Needle Aspirates from Non-Small-Cell Lung Cancer by RT-qPCR (CAT#: STEM-MT-0016-LGZ)
Analysis of OBSCN Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0391-LGZ)
Analysis of RMDN2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1755-LGZ)
Analysis of KCNJ9 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1081-LGZ)
Analysis of AMMECR1L Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1709-LGZ)
Analysis of ATAD3B Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1239-LGZ)
Analysis of SCN1A-AS1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1487-LGZ)
Analysis of Suid Herpesvirus-1 (SuHV-1) by Real-Time PCR Method (CAT#: STEM-MB-3446-LGZ)