Analysis of MPC2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0458-LGZ)

Introduction

Official Full Name: mitochondrial pyruvate carrier 2<br />Also known as: BRP44; SLC54A2<br />Make the same protein binding activity. Expected to be involved in mitochondrial pyruvate transmembrane transport. Expected to act upstream or within the mitochondrial pyruvate acetyl-CoA biosynthesis process and actively regulate insulin secretion, participating in cellular responses to glucose stimulation. Located in the mitochondria.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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