Analysis of MPP4 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1694-LGZ)

Introduction

Official Full Name: MAGUK p55 scaffold protein 4<br />Also known as: DLG6; ALS2CR5<br />This gene encodes a member of the membrane-associated guanylate kinase (MAGUK) protein family, with an N-terminal PDZ domain, a central src homology 3 region (SH3), and a C-terminal guanylate kinase-like (GUK) domain. The protein is localized to the outer limiting membrane in the retina, and is thought to function in photoreceptor polarity and the organization of specialized intercellular junctions. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of LCE3B Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0412-LGZ)
Analysis of F11R Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0946-LGZ)
Analysis of VCP Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2038-LGZ)
Analysis of Pythium Insidiosum by Real-Time PCR Method (CAT#: STEM-MB-3425-LGZ)
Analysis of Listeria Ivanovii by Real-Time PCR Method (CAT#: STEM-MB-4035-LGZ)
Analysis of Chryseobacterium Meningsepticum by Real-Time PCR Method (CAT#: STEM-MB-2659-LGZ)
Analysis of SUSD4 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0700-LGZ)
Analysis of Bovine Rhinitis B virus (BRBV) by Real-Time PCR Method (CAT#: STEM-MB-2750-LGZ)