Analysis of MTHFR Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0078-LGZ)

Introduction

The protein encoded by this gene catalyzes the conversion of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate, a co-substrate for the remethylation of homocysteine to methionine. Genetic variation in this gene affects susceptibility to occlusive vascular disease, neural tube defects, colon cancer, and acute leukemia, and mutations in this gene have been associated with methylenetetrahydrofolate reductase deficiency.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

methotrexate response - Toxicity, methotrexate response - Metabolism/PK

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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