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Analysis of MUC1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0406-LGZ)

Introduction

Official Full Name: mucin 1, cell surface associated<br />Also known as: EMA; MCD; PEM; PUM; KL-6; MAM6; MCKD; PEMT; CD227; H23AG; MCKD1; MUC-1; ADMCKD; ADTKD2; Ca15-3; ADMCKD1; CA 15-3; MUC-1/X; MUC1/ZD; MUC-1/SEC<br />This gene encodes a membrane-bound protein, a member of the mucin family. Mucin is an O-glycosylated protein that plays an important role in forming a protective mucosal barrier on epithelial surfaces. These proteins also play a role in intracellular signaling. The protein is expressed on the apical surface of epithelial cells, which line the mucosal surface of many different tissues, including lung, breast, stomach and pancreas. The protein is proteolytically cleaved into α and β subunits, forming a heterodimeric complex. The N-terminal α subunit is involved in cell adhesion and the C-terminal β subunit is involved in cell signaling. Overexpression, abnormal intracellular localization, and changes in glycosylation of this protein are associated with cancer. This gene is known to contain a highly polymorphic variable number tandem repeat (VNTR) domain. Alternate splicing results in multiple transcript variants.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements