Analysis of Murine Norovirus (MNV) by RT-qPCR (CAT#: STEM-MT-0063-LGZ)

Introduction

Murine norovirus (MNV) is considered the most prevalent viral pathogen in captive mouse populations. The MNV rapid detection test will be a useful tool for monitoring and preventing MNV infection.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Virus Detection

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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