Analysis of Murine Norovirus (MNV) by RT-qPCR (CAT#: STEM-MT-0063-LGZ)
Introduction
Murine norovirus (MNV) is considered the most prevalent viral pathogen in captive mouse populations. The MNV rapid detection test will be a useful tool for monitoring and preventing MNV infection.
Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.
Applications
Virus Detection
Procedure
1. Sample processing and preparation of PCR reaction system. 2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument. 3. Set the program for PCR amplification. 4. Data analysis.
Materials
Sample: depends on the customer's analysis requirements