Analysis of Myeloperoxidase (MPO) Activity by Enzyme-labeled Instrument (CAT#: STEM-MB-1420-LGZ)

Introduction

Sensitivity: 19.42 U/L
Detection range: 19.42-893.31 U/L
Precision: inter-lot difference of 7.3%, intra-lot difference of 5.4%
Detection equipment: Enzyme-labeled Instrument
Detection wavelength: 450-470 nm

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Principle Applications Procedure Materials Notes Related Products

Principle

Myeloperoxidase reduces hydrogen peroxide to a complex, which generates yellow products by hydrogen donor o-anisidine. The product production is measured by colorimetry at 460 nm to calculate the activity of MPO.

Applications

It is suitable for detecting MPO activity in serum, plasma, milk and animal tissue samples.

Procedure

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

Materials

• Sample Type: serum, plasma, milk and animal tissue samples

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