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Analysis of Myl3/CMLC1 (Mouse) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0446-LGZ)

Introduction

MYL3 encodes myosin light chain 3, a base light chain also known in the literature as the ventricular isoform and the slow skeletal muscle isoform. Mutations in MYL3 have been identified as a cause of middle-left ventricular hypertrophic cardiomyopathy.




Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Cardiac Biomarkers, Cardiac Injury, Muscle Injury, Toxicology, Cardiovascular

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum
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