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Analysis of N-Cadherin (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0450-LGZ)

Introduction

N-cadherin (CDH2), also known as cadherin-2 (CDH2) or neural cadherin (NCAD), is a protein encoded by the CDH2 gene in the human body. N-cadherin is a transmembrane protein expressed in various tissues and functions to mediate cell-cell adhesion. In cardiac muscle, N-cadherin is a component of adherens junctions located on intercalated discs, which function to mechanically and electrically link neighboring cardiomyocytes.




Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Cardiac Biomarkers, Cardiovascular

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum
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