Analysis of NADP+/NADPH by Enzyme-labeled Instrument (CAT#: STEM-MB-1450-LGZ)

Glucose-6-phosphate (G6P) is oxidized to form 6-phosphogluconolactone (6-PG) under the action of glucose-6-phosphate dehydrogenase (G6PDH). In this reaction, NADP+ is reduced to NADPH; The resulting NADPH reduces WST-8 to orange-yellow formazan with an electron-coupled reagent 1-mPMS, which has a maximum absorption peak around 450 nm. The formazan produced in the reaction system is proportional to the total amount of NADP+ and NADPH in the sample.

It was used to detect the content, ratio and total content of NADP+(oxidized coenzyme Ⅱ) and NADPH(reduced coenzyme Ⅱ) in animal tissue and cell samples.

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

• Sample Type: animal tissue and cell samples