Analysis of NAP-2/CXCL7 (Human) by ELISA (CAT#: STEM-MB-1085-LGZ)

Introduction

Neutrophil-activating peptide 2 (NAP2) is the carboxy-terminal fragment of platelet basic protein (PBP), present in human platelet alpha granules. NAP-2 is one of the CXC chemokines. Similar to other ELR domain-containing CXC chemokines such as IL-8 and GRO proteins, NAP-2 has been shown to bind CXCR-2 and chemoattract and activate neutrophils. Although CTAP-III, β-TG and PBP represent N-terminally extended variants of NAP-2 and share the same CXC chemokine domain, these proteins do not exhibit NAP-2 activity. Recently, it was shown that the additional amino-terminal residues of CTAP-III mask the critical ELR receptor-binding domain exposed on NAP-2, which may be responsible for the lack of NAP-2 activity.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Cytokines & Chemokines, Immunology/Inflammation

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or other biological fluids

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