Analysis of NBPF3 Gene (Mutation) in Hereditary Breast Ovarian Cancer Syndrome by RT-qPCR (CAT#: STEM-MT-0087-LGZ)

Introduction

Also known as: AE2
The gene is a member of the neuroblastoma breakpoint family (NBPF), which consists of dozens of recently duplicated genes, mainly located in segments on human chromosome 1. This gene family has undergone its greatest expansion in the human lineage and, to a lesser extent, in primates. Members of this gene family are characterized by tandem duplicate copies of the DUF1220 protein domain. Most of the DUF1220 domains are located in the region of human chromosome 1q21.1, where DUF1220 copy number variations are associated with many developmental and neurogenetic disorders, such as microcephaly, macrocephaly, autism, schizophrenia, cognitive impairment, congenital heart disease, neuroblastoma, and congenital kidney and urinary tract abnormalities. Altered expression of some gene family members has been linked to several cancers. This gene family contains many pseudogenes.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Hereditary breast ovarian cancer syndrome

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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