Analysis of NDRG1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1861-LGZ)

Introduction

Official Full Name: N-myc downstream regulated 1
Also known as: GC4; RTP; DRG1; NDR1; NMSL; TDD5; CAP43; CMT4D; DRG-1; HMSNL; RIT42; TARG1; PROXY1
This gene is a member of the N-myc downregulated gene family and belongs to the α/β hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein involved in stress response, hormone response, cell growth and differentiation. The encoded protein is required for p53-mediated caspase activation and apoptosis. Mutations in this gene are one cause of peronealgia type 4D, and expression of this gene may be a prognostic indicator in several cancers. Alternative splicing transcript variants encoding multiple isoforms have been observed for this gene.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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