Analysis of NEU2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1762-LGZ)

Introduction

Official Full Name: neuraminidase 2
Also known as: SIAL2
This gene belongs to a family of glycohydrolytic enzymes which remove sialic acid residues from glycoproteins and glycolipids. Expression studies in COS7 cells confirmed that this gene encodes a functional sialidase. Its cytosolic localization was demonstrated by cell fractionation experiments.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of MIR2467 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1638-LGZ)
Analysis of DENND2C Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1147-LGZ)
Analysis of Aspergillus Spp. by Real-Time PCR Method (CAT#: STEM-MB-3260-LGZ)
Analysis of Schistosoma Bovis by Real-Time PCR Method (CAT#: STEM-MB-3368-LGZ)
Analysis of Aspergillus Nidulans by Real-Time PCR Method (CAT#: STEM-MB-2872-LGZ)
Analysis of INS-IGF2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2263-LGZ)
Analysis of Prevotella Spp. by Real-Time PCR Method (CAT#: STEM-MB-3804-LGZ)
Analysis of Varroa Spp. by Real-Time PCR Method (CAT#: STEM-MB-3548-LGZ)