Analysis of Neuropilin-1 (Human) by ELISA (CAT#: STEM-MB-1096-LGZ)

Introduction

Neuropilins (NRPs) are single-transmembrane, non-tyrosine kinase surface glycoproteins that exist in all vertebrates and are highly conserved across species. Two homologous NRP isoforms, NRP1 and NRP2, encoded by different genes, are known to exist, and these two NRPs were originally discovered as neuronal adhesion molecules. They were later found to be related to vascular biology, and normal embryonic vascular development requires NRP1 and NRP2 to participate in the formation of small lymphatic vessels and capillaries. Studies in the past 10 years have shown that NRP is a multifunctional protein involved in various biological processes besides neural and vascular development, among which NRP1 plays a major role in immunity and tumorigenesis.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Oncology & Cancer, Immunology/Inflammation

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Cell culture supernatant, Cell Lysate, EDTA Plasma, Hep Plasma, Serum

Other recommended products

Analysis of Plant ω-3fatty acid desaturases (ω-3FADS) by ELISA (CAT#: STEM-MB-2310-LGZ)
Analysis of ST/5-HT(Serotonin/5-Hydroxytryptamine) by ELISA (CAT#: STEM-MB-1667-LGZ)
Analysis of Serpin E1 (active) (Human) by ELISA (CAT#: STEM-MB-1181-LGZ)
Analysis of IGFBP-1 (Human) by ELISA (CAT#: STEM-MB-0917-LGZ)
Analysis of S100B (Human) by ELISA (CAT#: STEM-MB-1164-LGZ)
Analysis of Plant ω-Fatty Acid Desaturases (ω-FADS) by ELISA (CAT#: STEM-MB-2308-LGZ)
Analysis of Human FGL1(Fibrinogen Like Protein 1) by ELISA (CAT#: STEM-MB-1806-LGZ)
Analysis of Rat MAOA(Monoamine Oxidase Type A) by ELISA (CAT#: STEM-MB-2136-LGZ)