Analysis of NOC2L Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1352-LGZ)

Introduction

Official Full Name: NOC2 like nucleolar associated transcriptional repressor<br />Also known as: NIR; NET7; NET15; PPP1R112<br />Histone modification by histone acetyltransferases (HAT) and histone deacetylases (HDAC) can control major aspects of transcriptional regulation. NOC2L represents a novel HDAC-independent inhibitor of histone acetyltransferase (INHAT).

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of Campylobacter Colic by Real-Time PCR Method (CAT#: STEM-MB-3901-LGZ)
Analysis of Herba Cirsii Japonici by Real-Time PCR Method (CAT#: STEM-MB-4007-LGZ)
Analysis of SCGB1C1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2123-LGZ)
Analysis of Carnation Vein Mottle Virus(CVMV) by Real-Time PCR Method (CAT#: STEM-MB-2678-LGZ)
Analysis of HDAC4 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1559-LGZ)
Analysis of Oxyspirura Mansoni by Real-Time PCR Method (CAT#: STEM-MB-3098-LGZ)
Analysis of TMEM167B Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1210-LGZ)
Analysis of KRTCAP2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1100-LGZ)