Analysis of Non-esterified Free Fatty Acids (NEFA/FFA) by Ultraviolet-visible Spectrophotometer (CAT#: STEM-MB-1339-LGZ)

Introduction

Sensitivity: 0.05 mmol/L
Detection range: 0.05-2.0 mmol/L
Precision: inter-lot difference of 6.1%, intra-lot difference of 2.2%
Detection equipment: Ultraviolet-visible Spectrophotometer
Detection wavelength: 715 nm

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Principle Applications Procedure Materials

Principle

Under weakly acidic conditions, free fatty acids react with copper salts to produce copper soap, which has a characteristic absorption peak at 715 nm. The content of free fatty acids has a linear relationship with the degree of color development within a certain range, and the content of free fatty acids can be calculated.

Applications

It is suitable for the determination of free fatty acid content in serum and animal tissue.

Procedure

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

Materials

• Sample Type: serum and animal tissue

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