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Analysis of NR1I3 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0501-LGZ)

Introduction

Official Full Name: nuclear receptor subfamily 1 group I member 3<br />Also known as: CAR; CAR1; MB67<br />This gene encodes a member of the nuclear receptor superfamily, a key regulator of exogenous and endogenous metabolism. The protein binds to DNA in the form of a monomer or heterodimer with the retinoid X receptor, and regulates the transcription of target genes involved in drug metabolism and bilirubin clearance, such as members of the cytochrome P450 family. Unlike most nuclear receptors, this regulator of transcription is constitutively active in the absence of ligand but is regulated by both agonists and inverse agonists. Ligand binding causes the protein to translocate to the nucleus where it activates or represses the transcription of target genes. These ligands include bilirubin, various exotic compounds, steroid hormones, and prescription drugs. In addition to drug metabolism, CAR proteins have also been reported to regulate genes involved in glucose metabolism, lipid metabolism, cell proliferation, and circadian clock regulation. Multiple transcript variants encoding different isoforms have been found for this gene.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements