Analysis of NR2F1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1842-LGZ)

Introduction

Official Full Name: nuclear receptor subfamily 2 group F member 1<br />Also known as: EAR3; BBOAS; EAR-3; SVP44; BBSOAS; ERBAL3; COUPTF1; TFCOUP1; COUP-TFI; TCFCOUP1<br />The protein encoded by this gene is a nuclear hormone receptor and transcriptional regulator. The encoded protein acts as a homodimer and binds to 5'-AGGTCA-3' repeats. Defects in this gene are a cause of Bosch-Boonstra optic atrophy syndrome (BBOAS).

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of MPZ Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0156-LGZ)
Analysis of MAP3K21 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0404-LGZ)
Analysis of GPR153 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1332-LGZ)
Analysis of MIR6759 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2501-LGZ)
Analysis of TMEM183A Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0729-LGZ)
Analysis of NTMT2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0498-LGZ)
Analysis of Porcine Enteric Calicivirus (PECV) by Real-Time PCR Method (CAT#: STEM-MB-3205-LGZ)
Analysis of Canine Parvo Virus(CPV) by Real-Time PCR Method (CAT#: STEM-MB-2688-LGZ)