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Analysis of NRAS Gene (Mutation) in Noonan Syndrome by RT-qPCR (CAT#: STEM-MT-0129-LGZ)

Introduction

Also known as: NS6; CMNS; KRAS; NCMS; ALPS4; N-ras; NRAS1
This is an N-ras oncogene that encodes a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated by palmitoylation and depalmitoylation of the ZDHHC9-GOLGA7 complex. The encoded protein has intrinsic GTPase activity, is activated by guanine nucleotide exchange factors, and is inactivated by GTPase activating proteins. Mutations in this gene have been associated with somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myeloid leukemia.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Noonan syndrome and Noonan-related syndrome

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements
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