Analysis of NT-proBNP (Rat) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0466-LGZ)

Introduction

Detection of serum markers is one of the important means of early diagnosis and prognosis of heart failure. Among them, natriuretic peptide (a marker of activation of neuroendocrine system), including cerebrouretic natriuretic peptide (BNP) and amino terminal cerebral natriuretic peptide (NT-proBNP), is the preferred serum marker for heart failure recommended by both domestic and foreign heart failure guidelines (ECS/ACC/AHA/HFSA/CSC).

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Principle Applications Procedure Materials Notes Related Products

Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Cardiac Biomarkers, Cardiac Injury, Natriuretic Peptide, Toxicology, Cardiovascular

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum

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