Analysis of NUCB2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2414-LGZ)

Introduction

Official Full Name: nucleobindin 2
Also known as: NEFA; HEL-S-109
This gene encodes a protein with a suggested role in calcium level maintenance, eating regulation in the hypothalamus, and release of tumor necrosis factor from vascular endothelial cells. This protein binds calcium and has EF-folding domains.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Fast cycling PCR (CAT#: STEM-MB-0203-WXH)
Analysis of SYCP1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1165-LGZ)
Analysis of Hanzalova Virus by Real-Time PCR Method (CAT#: STEM-MB-3022-LGZ)
Analysis of CFAP74 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1279-LGZ)
Analysis of Herba Scutellariae Barbatae by Real-Time PCR Method (CAT#: STEM-MB-4006-LGZ)
Analysis of AIV-H7 Ag by Real-Time PCR Method (CAT#: STEM-MB-2904-LGZ)
Analysis of DNPEP Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1523-LGZ)
Analysis of ANO5 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2463-LGZ)