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Analysis of NUP133 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0211-LGZ)

Introduction

Also known as: GAMOS8; NPHS18; hNUP133<br />In eukaryotic cells, the nuclear membrane forms distinct nuclear and cytoplasmic compartments. It consists of two concentric membranes perforated by nuclear pores, large protein complexes that form water channels to regulate the flow of macromolecules between the nucleus and cytoplasm. These complexes are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. The nucleoporin encoded by this gene exhibits evolutionarily conserved interactions with other nucleoporins. The protein localizes to both sides of the interphase nuclear pore complex, maintains association with the complex during mitosis, and targets the reorganized nuclear membrane during early stages. This protein is also localized to the centromere of mitotic cells.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements