Analysis of OR10Z1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0152-LGZ)

Introduction

Also known as: OR1-15<br />Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Hereditary spherocytosis type 3, Elliptocytosis 2, Pyropoikilocytosis, hereditary

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of SNHG28 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0675-LGZ)
Analysis of Oncorhynchus Masou Virus (OMV) by Real-Time PCR Method (CAT#: STEM-MB-3794-LGZ)
Analysis of TACC1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1878-LGZ)
Analysis of FH Gene (Mutation) in Fumarase Deficiency by RT-qPCR (CAT#: STEM-MT-0193-LGZ)
Analysis of IWS1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1696-LGZ)
Analysis of Eimeria Spp. by Real-Time PCR Method (CAT#: STEM-MB-3624-LGZ)
Analysis of OR52B4 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2344-LGZ)
Analysis of LOC121009634 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1601-LGZ)