Analysis of Organophosphorus Agrochemical Adsorption in MOFs by Isothermal Titration Calorimetry (ITC) (CAT#: STEM-MB-2543-LGZ)

Introduction

The expansion of manufacturing and commercial agriculture, as well as rapid globalization, has led to widespread contamination of freshwater supplies with chemical toxins, including persistent organic pollutants. Effective mitigation of this pollution is essential to protect human health, animal and aquatic life, and the environment. Adsorption is currently the most economically viable strategy for water purification. Owing to their crystalline and modular nature, metal-organic frameworks (MOFs) are excellent platform materials for systematically studying the physical and chemical properties governing adsorption processes.

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Principle Applications Procedure Materials Notes Related Products

Principle

A reactant is placed in a temperature-controlled sample cell and coupled to the reference cell through a thermocouple loop. The sample cell and the reference cell are in the same external environment. A specific titration agent (selected as required by the test) is added to the sample cell quantitatively. The change in energy in response can be sensitively detected and triggered by a positive or negative feedback thermostat to keep the temperature constant.

Applications

For characterizing thermodynamic parameters of biomolecular interactions.

Procedure

1. Preparation before the experiment.
2. Add 40ul of ligand or small molecule protein solution to the titration syringe, 200ul of large molecule protein solution to the sample pool, and 200ul of distilled water to the reference pool.
3. Set Experimental Parameters in the interface.
4. In the set up interface, set the storage path of experimental data and experimental methods, as well as the current user.
5. Put the titration syringe into the sample pool and click Start to start the experiment. Display real-time images of the experiment on the Real Time Plot interface.

Materials

• Sample Type: protein 50 micrograms, small molecules 10mM/500ul, the higher the sample concentration is the better, protein peptides need to be sent at low temperature.

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