Analysis of Osteoprotegerin (Human) by ELISA (CAT#: STEM-MB-1109-LGZ)

Introduction

Osteoprotegerin (OPG) is a member of the tumor necrosis factor (TNF) receptor superfamily, also known as TNFRS11B or OCIF, which belongs to type II transmembrane protein. OPG is mainly expressed by bone marrow stromal cells. In addition, its expression has also been detected in the immune system (such as lymph nodes, B cells and dendritic cells (DCs)) and the vascular system (such as the heart, arteries and veins). Usually, the expression of OPG is closely related to some bone diseases. But more evidence shows that OPG can promote cell survival by inhibiting TNF-related apoptosis. In addition, some in vitro, in vivo and clinical studies have also shown that OPG plays a role in tumors.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Bone Disorders

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Cell culture supernatant, Cell Lysate, Plasma, Serum

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