Analysis of Ovine Astrovirus (OAstV) by Real-Time PCR Method (CAT#: STEM-MB-3069-LGZ)

Introduction

The OAstV was first identified in 1977 in Scotland in lambs suffering from diarrhea shortly after the description of astroviruses in human. The OAstV was identified by electron microscopy and was purified and characterized structurally in 1981.
A real-time polymerase chain reaction (real-time PCR, or qPCR) is a laboratory technique of molecular biology based on the polymerase chain reaction(PCR). It monitors the amplification of a targeted DNA molecule during the PCR(i.e.,in real time), not at its end, as in conventional PCR.
Two common methods for the detection of PCR products in real-time PCR are(1)non-specific fluorescent dyes that intercalate with any double-stranded DNA and (2)sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter, which permits detection only after hybridization of the probe with its complementary sequence.