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Analysis of OX40/TNFRSF4 (Human) by ELISA (CAT#: STEM-MB-1110-LGZ)

Introduction

OX40 (CD134, TNFRSF4) was originally defined as a marker of T cell activation, and later discovered to be a member of the NGFR/TNFR superfamily with co-activation function. The OX40 gene is located on human chromosome 1 (mouse chromosome 4), encoding a 50kD type I transmembrane glycoprotein. The extracellular region has 191 amino acids and contains three complete and one shorter cysteine-rich domains (CRDs). Mainly expressed on activated effector T cells (Teffs) and regulatory T cells (Tregs), but also on NKT cells, NK cells and neutrophils.




Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Immunology/Inflammation

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Cell culture extracts, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
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