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Analysis of p38 (Human, Mouse, Rat) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0477-LGZ)

Introduction

p38 mitogen-activated protein kinase (p38MAPK), which first came to attention as a stress-related kinase, has recently been found to be involved in a variety of tumors, In cervical cancer, ovarian cancer, liver cancer and lymphoma, it is closely related to the initiation of apoptosis and the quiescence of cell cycle, and has cell specificity. In different tumor cells, it has different effects, or even completely opposite effects. Therefore, specific research on the role of p38MAPK signaling pathway in various tumors and normal cells can provide theoretical guidance for further tumor therapy.




Principle

Detection Technique: MSD Hypersensitive Multi-Factor Electrochemiluminescence

Capture Antibody:
Phospho-p38: Mouse Monoclonal
Total p38: Rabbit Polyclonal

Detection Antibody:
Phospho-p38: Rabbit Polyclonal
Total p38: Rabbit Polyclonal

Applications

Phosphoproteins, Intracellular Signaling

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Whole Cell Lysate
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