Analysis of P4HB/PO4DB/ERBA2L by ELISA (CAT#: STEM-MB-1535-LGZ)

Introduction

Protein disulfide isomerase, also known as proline 4-hydroxylase β subunit (P4HB), cellular thyroid hormone binding protein (p55) and glutathione-insulin transhydrogenase, is a kind of peripheral membrane protein belonging to the protein disulfide isomerase family. It contains two thioredoxin domains that catalyze the formation, breaking, and rearrangement of disulfide bonds. P4HB was located near the CD4 rich area on the surface of lymphocytes. In melansome components, P4HB reduces and activates the fusion properties of HIV-1 gp120 surface proteins, thereby allowing HIV-1 to enter the cell. P4HB acts as a chaperone, inhibiting the aggregation of misfolded proteins. P4HB may be involved with other molecular chaperones in the structural modification of TG precursors in hormone biosynthesis.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Protein Expression

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma, cell culture supernatant and other biological samples

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