Analysis of Parelaphostrongylus Tenius by Real-Time PCR Method (CAT#: STEM-MB-3800-LGZ)

Introduction

Parelaphostrongylus tenuis is a nematode parasite common to white tail deer. Sheep and goats, camelids, elk, caribou, and moose may be aberrant hosts. Adult worms in meningeal tissue lay eggs that develop into first stage larvae that migrate through the bloodstream to the lungs.<br />A real-time polymerase chain reaction (real-time PCR, or qPCR) is a laboratory technique of molecular biology based on the polymerase chain reaction(PCR). It monitors the amplification of a targeted DNA molecule during the PCR(i.e.,in real time), not at its end, as in conventional PCR.<br />Two common methods for the detection of PCR products in real-time PCR are(1)non-specific fluorescent dyes that intercalate with any double-stranded DNA and (2)sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter, which permits detection only after hybridization of the probe with its complementary sequence.