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Analysis of PBX1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0563-LGZ)

Introduction

Official Full Name: PBX homeobox 1<br />Also known as: CAKUHED<br />The nucleoprotein encoded by this gene belongs to the PBX homeobox transcription factor family. Studies in mice suggest that the gene may be involved in the regulation of bone formation and is required for bone patterning and programming. A chromosomal translocation t(1;19) involving this gene and the TCF3/E2A gene is associated with pre-B-cell acute lymphoblastic leukemia. The resulting fusion protein, in which the DNA-binding domain of E2A is replaced by that of this protein, transforms cells by constitutively activating transcription of genes regulated by the PBX protein family. Alternative splice transcript variants of this gene have been found to encode distinct isoforms.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements