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Analysis of PBXIP1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0562-LGZ)

Introduction

Official Full Name: PBX homeobox interacting protein 1<br />Also known as: HPIP<br />The protein encoded by this gene interacts with the PBX1 homeodomain protein, inhibiting its transcriptional activation potential by preventing its binding to DNA. The encoded protein is found primarily in the cytoplasm but can be shuttled into the nucleus where it also interacts with estrogen receptors to promote the proliferation of breast, brain and lung tumors. Several transcript variants of this gene have been found encoding different isoforms. Many more variants exist, but their full lengths have not been determined.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements