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Analysis of PDPN Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1374-LGZ)

Introduction

Official Full Name: podoplanin
Also known as: T1A; GP36; GP40; Gp38; OTS8; T1A2; TI1A; D2-40; T1A-2; AGGRUS; HT1A-1; PA2.26
This gene encodes a type I integral membrane glycoprotein with a diverse distribution in human tissues. The physiological function of this protein may be related to its mucus-type properties. Homologous proteins have been described in other species as differentiation antigens and influenza virus receptors. The exact function of this protein has not been determined, but it is considered a marker of lung damage. Alternative splicing transcript variants encoding different isoforms have been identified.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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