Analysis of Peptostreptococcus Anaerobius by Real-Time PCR Method (CAT#: STEM-MB-3557-LGZ)

Introduction

Peptostreptococcus anaerobius is a slow growing, non-spore forming, Gram-positive anaerobic cocci (GPAC) bacteria. This means that it is not able to grow in oxygen-rich environments. It is a short chain cocci, or spherical bacterium, with a size of 0.5µ to 0.6µ. During growth, they form a raised, white, glossy colony.
A real-time polymerase chain reaction (real-time PCR, or qPCR) is a laboratory technique of molecular biology based on the polymerase chain reaction(PCR). It monitors the amplification of a targeted DNA molecule during the PCR(i.e.,in real time), not at its end, as in conventional PCR.
Two common methods for the detection of PCR products in real-time PCR are(1)non-specific fluorescent dyes that intercalate with any double-stranded DNA and (2)sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter, which permits detection only after hybridization of the probe with its complementary sequence.