Analysis of Periostin/OSF-2 (Mouse) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0492-LGZ)

Introduction

Periostin (POSTN, PN or OSF-2) is an extracellular matrix secreted protein. Human Periostin protein contains 836 amino acids, molecular weight is about 93.3kDa, about 36kbp, located on chromosome 13q13.3. The human Periostin protein structure contains an amino-terminal secretory signal peptide, a cysteine-rich amino-terminus, four homologous repeat regions and a hydrophilic carboxyl-terminus.

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Principle Applications Procedure Materials Notes Related Products

Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Oncology & Cancer, Immunology/Inflammation, Neurobiology/Neurodegeneration

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum

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