Analysis of PGLYRP3 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0580-LGZ)

Introduction

Official Full Name: peptidoglycan recognition protein 3
Also known as: PGRPIA; PGRP-Ialpha; PGLYRPIalpha
This gene encodes a peptidoglycan recognition protein, which belongs to the N-acetylmuramoyl-L-alanine amidase 2 family. These proteins are part of the innate immune system and recognize peptidoglycan, a ubiquitous component of bacterial cell walls. This antimicrobial protein binds to murein peptidoglycans of Gram-positive bacteria.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of Mycoplasma Iowae by Real-Time PCR Method (CAT#: STEM-MB-3746-LGZ)
Analysis of Cochliomyia Hominivorax by Real-Time PCR Method (CAT#: STEM-MB-2630-LGZ)
Analysis of BRINP3 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0822-LGZ)
Analysis of Theileria Equi by Real-Time PCR Method (CAT#: STEM-MB-3001-LGZ)
Analysis of SKI Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1404-LGZ)
Analysis of Cortex Albiziae by Real-Time PCR Method (CAT#: STEM-MB-3967-LGZ)
Analysis of FLAD1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0973-LGZ)
Analysis of NECTIN4 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0494-LGZ)