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Analysis of PIDD1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2385-LGZ)

Introduction

Official Full Name: p53-induced death domain protein 1<br />Also known as: LRDD; PIDD; MRT75<br />The protein encoded by this gene contains a leucine-rich repeat and a death domain. This protein has been shown to interact with other death domain proteins, such as Fas (TNFRSF6)-associated via death domain (FADD) and MAP-kinase activating death domain-containing protein (MADD), and thus may function as an adapter protein in cell death-related signaling processes. Expression of the mouse counterpart of this gene has been positively regulated by the tumor suppressor p53 and induces apoptosis upon DNA damage, suggesting that this gene may be an effector of p53-dependent apoptosis. Alternative splicing results in multiple transcript variants.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements