Unlock Exclusive Discounts & Flash Sales! Click Here to Join the Deals on Every Wednesday!
Pigeon Poxvirus (PPV) was detected in eight pigeons suffering from wart like nodular lesions in two Egyptian governorates (Assiut and New Valley) during summer 2018.
A real-time polymerase chain reaction (real-time PCR, or qPCR) is a laboratory technique of molecular biology based on the polymerase chain reaction(PCR). It monitors the amplification of a targeted DNA molecule during the PCR(i.e.,in real time), not at its end, as in conventional PCR.
Two common methods for the detection of PCR products in real-time PCR are(1)non-specific fluorescent dyes that intercalate with any double-stranded DNA and (2)sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter, which permits detection only after hybridization of the probe with its complementary sequence.