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Analysis of PLA2G4A Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0568-LGZ)

Introduction

Official Full Name: phospholipase A2 group IVA<br />Also known as: GURDP; cPLA2; PLA2G4; cPLA2-alpha<br />This gene encodes a member of the group IV family of cytoplasmic phospholipase A2. This enzyme catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid, which is subsequently metabolized to eicosanoids. Eicosanoids, including prostaglandins and leukotrienes, are lipid-based cytokines that regulate hemodynamics, inflammatory responses, and other intracellular pathways. The hydrolysis reaction also produces lysophospholipids, which are converted to platelet-activating factor. The enzyme is activated by elevated intracellular Ca(2+) levels and phosphorylation, leading to its translocation from the cytoplasm and nucleus to perinuclear membrane vesicles. Alternative splicing results in multiple transcript variants.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements