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Analysis of PLEKHM2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1365-LGZ)

Introduction

Official Full Name: pleckstrin homology and RUN domain containing M2<br />Also known as: SKIP<br />This gene encodes a protein that binds the plus-end-directed microtubule motor protein kinase and the lysosomal GTPase AR18 and is required for the distribution of lysosomes away from the microtubule organizing center. The encoded protein belongs to the multisubunit block-1-associated complex that regulates lysosomal localization. It binds a Salmonella effector protein, termed Salmonella-induced filament a, which is a key host determinant of Salmonella pathogenesis. It has a domain architecture consisting of an N-terminal RPIP8, UNC-14, and NESCA (RUN) domain that binds kinesin-1 as well as the lysosomal GTPase Arl8, and a C-terminal pleckstrin homology domain that binds the Salmonella induced filament A effector protein. Naturally occurring mutations in this gene lead to abnormal localization of lysosomes, impaired autophagy flux and are associated with recessive dilated cardiomyopathy and left ventricular noncompaction.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements