Analysis of PPP1R15B Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0594-LGZ)

Introduction

Official Full Name: protein phosphatase 1 regulatory subunit 15B
Also known as: CREP; MSSGM2
This gene encodes a protein phosphatase I interacting protein that promotes dephosphorylation of eukaryotic translation initiation factor 2A to regulate translation under cellular stress conditions. The transcribed messenger RNA contains two upstream open reading frames (ORFs), which under normal conditions repress the translation of proteins mainly encoding ORFs, which are expressed at high levels in response to stress. Ongoing translation of mRNA under conditions of inactivation of eukaryotic translation initiation factor 2A is thought to create a feedback loop for gene reactivation during stress recovery. Furthermore, this protein has been shown to play a translation-independent role in membrane trafficking and is required for exocytosis in erythroleukemic cells. Allelic variation in this gene has been associated with microcephaly, short stature, and impaired glucose metabolism.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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