Analysis of PRDM16 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0073-LGZ)

Introduction

Also known as: MEL1; KMT8F; LVNC8; PFM13; CMD1LL<br />The reciprocal translocation t(1;3)(p36;q21) occurs in a subset of myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML). This gene is located near the 1p36.3 breakpoint and has been shown to be specifically expressed in the t(1:3)(p36,q21)-positive MDS/AML. The protein encoded by this gene is a zinc finger transcription factor and contains an N-terminal PR domain. The translocation results in the overexpression of a truncated version of this protein that lacks the PR domain, which may play an important role in the pathogenesis of MDS and AML. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Left ventricular noncompaction 8, Primary familial hypertrophic cardiomyopathy

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of LINC02898 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1734-LGZ)
Analysis of FIRRM Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0974-LGZ)
Hot Start PCR (CAT#: STEM-MB-0190-WXH)
Analysis of PAPSS2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2065-LGZ)
Analysis of SOX11 mRNA by RT-qPCR (CAT#: STEM-MT-0043-LGZ)
Suicide PCR (CAT#: STEM-MB-0220-WXH)
Analysis of NCSTN Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0466-LGZ)
Analysis of MIR34A Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1339-LGZ)