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Analysis of PRDX-6 (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0500-LGZ)

Introduction

Peroxiredoxin 6 (Prdx-6) belongs to the non-selenoperoxidase (prdx) family of antioxidant enzymes. It is the only member of the Prdx family that possesses both glutathione peroxidase and phospholipase A2 activities. Prdx-6 regulates phospholipid turnover and protects cells from oxidative damage. Prdx6 is expressed in all major organs, with particularly high expression levels in lung cells, and regulates lung surfactant phospholipid synthesis and metabolic turnover.




Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Neurobiology/Neurodegeneration, Oncology & Cancer

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum
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