Analysis of protein tertiary structure by Circular dichroism (CD) (CAT#: STEM-MB-0597-WXH)

Introduction

The CD spectrum of a protein in the 'near-UV' spectral region (250– 320 nm) can be sensitive to certain aspects of tertiary structure. At these wavelengths, the chromophores are the aromatic amino acids and disulfide bonds, and the CD signals they produce are sensitive to the overall tertiary structure of the protein. The near UV CD spectrum of a protein provides a valuable fingerprint of the tertiary structure of that protein, which can be used to compare, for example, wild type and mutant forms of proteins.