Analysis of PROX1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0587-LGZ)

Introduction

Official Full Name: prospero homeobox 1<br />The protein encoded by this gene is a member of the homeobox family of transcription factors. Members of this family contain a homeobox domain, a helix-helix-helix structure of 60 amino acids that binds DNA and RNA. The protein encoded by this gene is conserved in vertebrates and may play an important role in development. Altered levels of this protein have been reported in cancers of different organs, such as colon, brain, blood, breast, pancreas, liver and esophagus. Alternative splicing results in multiple transcript variants.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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